alexa fluor 647 anti human cd30 (Novus Biologicals)
Structured Review

Alexa Fluor 647 Anti Human Cd30, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alexa+fluor+647+anti+human+cd30/Human+CD30+Ligand%2FTNFSF8+Alexa+Fluor%C2%AE+647-conjugated+Antibody/pmc08534096-51-26-32
Average 91 stars, based on 1 article reviews
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1) Product Images from "Landscape of 4D Cell Interaction in Hodgkin and Non-Hodgkin Lymphomas"
Article Title: Landscape of 4D Cell Interaction in Hodgkin and Non-Hodgkin Lymphomas
Journal: Cancers
doi: 10.3390/cancers13205208
Figure Legend Snippet: Number of cells analyzed in the different cases.
Techniques Used:
Figure Legend Snippet: Velocity of different cell types under reactive and neoplastic conditions. ( a ). Velocity of PD1-positive T cells, CD20-positive B cells and CD30-positive cells in all cases studied (*** p < 0.001, one-way-ANOVA with Bonferroni’s post-test for multiple comparisons). Each dot represents the mean from one movie. ( b ). Velocity of CD20-positive B cells in lymphadenitis and malignant lymphomas. The velocity of bulk CD20-positive B cells in CD20+ lymphomas largely represents the velocity of tumor cells, whereas the velocity in CD20- lymphomas corresponds to reactive bystander B cells. (** p = 0.0042, unpaired t -test.) Each dot represents the mean from one movie. ( c ). Velocity of CD20-positive B cells listed according to different lymphoma entities. No significant differences were observed. Each dot represents the mean from one movie. Lymphadenitis: 4 cases, AITL, DLBCL and MCL: one case, MGZL and NLPHL: two cases, cHL: three cases. ( d ). Velocity of PD1-positive T cells grouped according to diagnosis of lymphadenitis, mature B-cell lymphomas and Hodgkin lymphomas. Each dot represents the mean from one movie. * p < 0.05, ** p < 0.01, Kruskal–Wallis test with Dunn’s post-test for multiple comparisons. ( e ). Velocity of PD1-positive T cells according to lymphoma entities. ** p < 0.01, *** p < 0.001, Kruskal–Wallis-Test with Dunn´s post-test for multiple comparisons. Each dot represents the mean from one movie. Lymphadenitis: 5 cases, AITL and MCL: one case, MGZL, DLBCL, FL and NLPHL: two cases, cHL: three cases. ( f ). Velocity of CD30-positive lymphoma cells compared with CD30-positive reactive bystander cells in CD30-negative lymphomas and lymphadenitis (* p < 0.05, unpaired t -test). Each dot represents the mean from one movie.
Techniques Used: Biomarker Discovery
Figure Legend Snippet: Cell tracks according to different lymphoma types superimposed on fluorescence images. ( a – c ) Tracks of the tumor cells of mantle cell lymphoma ( a ), NLPHL ( b ) and MGZL ( c ) in CD20- or CD30-immunostaining, as indicated. Color of tracks corresponds to the observed velocity as indicated in the scale. ( d – f ) Tracks of the bystander T cells of the same cases: mantle cell lymphoma ( d ), NLPHL ( e ) and MGZL ( f ) in PD1-staining. Color of tracks corresponds to the observed velocity as indicated in the scale. ( g ) HE staining (200x) of the mantle cell lymphoma from ( a , d ). ( h ) HE staining (200x) of the NLPHL from ( b , e ). ( i ) HE staining (200×) of the MGZL from ( c , f ).
Techniques Used: Fluorescence, Immunostaining, Staining
Figure Legend Snippet: Cell–cell contacts between tumor and bystander cells. ( a ) Number of cell–cell contacts per movie between tumor cells (CD20 or CD30 as indicated) and PD1-positive T cells (for AITL PD1-positive tumor cells and CD20-positive B cells). Each dot represents the mean from one movie. Lymphadenitis: 4 cases, AITL, DLBCL, NLPHL and MCL: one case, MGZL: two cases, cHL: three cases. ( b ) Duration of cell–cell contacts between tumor (CD20 or CD30 as indicated) and PD1-positive T cells (for AITL PD1-positive tumor cells and CD20-positive B cells, * p < 0.05, Kruskal–Wallis test with Dunn’s post-test for multiple comparisons). Each dot represents the mean from one movie. Lymphadenitis: 4 cases, AITL, DLBCL, NLPHL and MCL: one case, MGZL: two cases, cHL: three cases.
Techniques Used:
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Staining:Article Title: Landscape of 4D Cell Interaction in Hodgkin and Non-Hodgkin Lymphomas Article Snippet: .. Live vibratome sections were stained for 15 min at 37 °C with the following antibodies: Alexa Fluor 647–anti-human PD-1 (clone EH12.2H7; BioLegend, San Diego, CA, USA), |